mikron 71 fluorimeter

The smallest fluorescence detector for liquid chromatography with changeable light sources and filters for a number of substances

As a detector in the analytical laboratory or as one of many measurement channels in SMB systems in process chromatography and for other fluorimetric applications. High sensitivity by means of the exact regulation of the light source via the reference channel. A combination of excitation light source and emission filter can be achieved by various methods. One measuring cell.

Product advantages

small

Regardless of where you take measurements, mikron 71 is right on the spot. With a diameter of 32 mm (1.25 in.) and only 128 mm (5 in.) in length, it fits perfectly in every position, e.g. fixed to the side of your system. There is no smaller fluo­re­scence detector for HPLC.

dynamic

You need high sensitivity and a large dynamic range of five orders of magnitude?
Use mikron 71. It measures at 100 Hz and sees changes in concentration quickly with a time constant of 0.1 s. Thanks to the reference channel it is extremely precise.

cool

A mikron 71 can be ope­ra­ted in cooled sur­roun­dings at 3 °C (37 °F).
Because of its small size it can be put com­ple­te­ly into a re­fri­ge­ra­tor. And it stays cold while being measured –
Just what you need for bio­che­mi­cal app­li­ca­tions.

bio-compatible

Only the cuvette from quartz glass and the PEEK seals are wetted, both being chemically resistant and biologically inert materials.
With PEEK being provided on request in USP Class VI, which moreover is TSE-free, your total process chain is documented compliantly.

open

mikron 71 speaks in many ways – on the hard­ware side via USB-C or via industry standard RS-485.
We have drivers for all current programs. More­over there is the open Runge protocol for your own im­ple­men­ta­tion.

modular

The mikron 71 is the detector for routine tasks, which can vary. According to your wishes the tasks can be aligned, with light sources and filters for different substances. Your res­pon­si­bi­li­ties are changing? No problem: you take the mikron 71 with you.

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Basic device

Detector

Design type	Filter fluorimeter with LED light source
Sensitivity	limit of detection	100 pmol/l (1)
	dynamical range	105/103 (2)
	noise	< 0,1 % (3)
Signal	sampling rate	1 ... 100 Hz
	channels	1   with reference channel
Interfaces	USB-C or RS-485 (data, energy)
Power	electrical	< 2.5 W ( < 500 mA at 5 V)
Dimensions		Ø32 mm × 128 mm (1.25 × 5 in.)
	weight	160 g   (5.6 oz.)
Protocol	open, text-based
Drivers	DataApex Clarity, SCPA PrepCon, LabView (4)
Markings	CE, UKCA

⁽¹⁾ fluorescein in PBS, λ = 490/520 nm
⁽²⁾ with/without switching the gain
⁽³⁾ without gain
⁽⁴⁾ All registered trademarks are the property of their respective owners.

Light sources and filter sets

Light source	bandwidth	10 nm
	accuracy	±2,5 nm
	precision	±1 nm
Filter	bandwidth	50 nm
Features	automatic recognition
	operating hours counter (LED)
Delivery programme	substance	excitation/emission
	anthracene	340/450 nm
	aflatoxin (5), NADH	360/450 nm
	Alexa Fluor 350 (4)	360/450 nm
	CFP	430/480 nm
	fluorescein, GFP	490/520 nm
	5-TAMRA	530/580 nm

⁽⁵⁾ aflatoxin B1, B2, G1, G2, M1, M2
other wavelengths on enquiry
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Measuring cell

analytical 9 µl

cuvette	di = 1.2 mm
volume	9 µl
ports	1/4″-28 UNF
	flat
cell body	PEEK
wetted	fused silica
	PEEK (8)
flow rate	1,000 ml/min
max. press.	5 bar (72 psi)

⁽⁸⁾ also as USP class VI, TSE-free

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